Framework

The theoretical basis and operational framework of efc software are shown in Figure 3. The theories and models involved in efc software are divided into the theories and models in pre-processing stage and in data analysis stage. The former mainly involves the blank subtraction of EEM dataset, correction of the inner filter effect, the normalization using water Raman scan, calibration with quinoline sulfate, and elimination of Raman & Rayleigh scattering. The latter mainly involves absorption spectrum index, fluorescence spectrum index, PARAFAC pre-analysis, outliers' omitting, models' optimization and validation, and models' comparison and fitting.

Figure 3 The theoretical basis and operational framework of efc software